Tottenham boss Mauricio Pochettino said his side were suffering from “stress and fatigue” after they slipped to defeat at home to West Ham in the Premier League.
Michail Antonio’s superb second-half strike was enough to inflict Spurs’ first loss after five games in their new stadium.
Spurs face Ajax in the first leg of the Champions League semi-finals on Tuesday, their eighth game in April.
Third-placed Spurs had won all four of their games since moving into Tottenham Hotspur Stadium this month.
Antonio’s goal in the 67th minute was the first they had conceded at home since moving into their new stadium and means they are not yet certain of finishing in the top four and securing Champions League football next season.
The victory ended a run of four games without a win for West Ham, who remain 11th in the table with two games to play this season.
Meanwhile, Southampton manager Ralph Hasenhuttl praised his side’s “amazing” achievement after an entertaining draw with Bournemouth secured their Premier League survival.
The Saints were 18th with only nine points from 15 games when Hasenhuttl replaced the sacked Mark Hughes in December.
Shane Long gave the hosts a deserved 12th-minute lead with a shot that took a deflection off of Nathan Ake following Nathan Redmond’s pass.
But Bournemouth equalised with their first shot of the match when Dan Gosling finished off a quick counter-attack after being set up by Callum Wilson.
Long then could only hit the post with an open goal before Wilson put Bournemouth ahead from a rebound as Angus Gunn could not hold on to David Brooks’ effort.
However, Saints fought back and James Ward-Prowse’s low strike made it 2-2 before substitute Matt Targett headed the hosts in front from Yan Valery’s right-wing cross.
But Wilson grabbed his second – and Bournemouth’s third – late on, bundling the ball home from close range after Ryan Fraser’s run
Finally, Wolves moved four points clear of Watford in the race for seventh place in the Premier League as goals from Raul Jimenez and Diogo Jota helped Nuno Espirito Santo’s side earn all three points at Vicarage Road.
Jimenez opened the scoring with his 13th league goal of the season, turning Jota’s low cross past Ben Foster from point-blank range.
Andre Gray capitalised on an error from Ryan Bennett to equalise for Watford shortly after the restart, but a mistake from Foster allowed Wolves to retake the lead with 13 minutes remaining.
Foster misjudged Ruben Neves’ long ball into the Watford penalty area, allowing Jota to tap into the unguarded net to seal the win for the visitors.
The result lifts Wolves on to 54 points, while Watford drop to ninth.